h3.3 antibody Search Results


h3 3 epicypher  (EpiCypher)
93
EpiCypher h3 3 epicypher
H3 3 Epicypher, supplied by EpiCypher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/H3%2E3+Antibody%2C+SNAP-Certified+for+CUT%26RUN/bio_rxiv__2025__11__19__685761-300-15-16
Average 93 stars, based on 1 article reviews
h3 3 epicypher - by Bioz Stars, 2026-11
93/100 stars
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anti h3k27me  (Rockland Immunochemicals)
94
Rockland Immunochemicals anti h3k27me
Anti H3k27me, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+K27+methyl+Antibody/pmc12757043-233-21-22
Average 94 stars, based on 1 article reviews
anti h3k27me - by Bioz Stars, 2026-11
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anti h3  (Proteintech)
93
Proteintech anti h3
Anti H3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/H3F3B+Antibody/pmc11186203-58-37-39
Average 93 stars, based on 1 article reviews
anti h3 - by Bioz Stars, 2026-11
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histone h3  (Proteintech)
94
Proteintech histone h3
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Histone H3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3%2E3+Antibody/pm39374547-66-25-28
Average 94 stars, based on 1 article reviews
histone h3 - by Bioz Stars, 2026-11
94/100 stars
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rabbit anti ph3  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit anti ph3
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Rabbit Anti Ph3, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+K4-Me3%2FK9-Ac+Antibody/pmc09094666-312-105-134
Average 93 stars, based on 1 article reviews
rabbit anti ph3 - by Bioz Stars, 2026-11
93/100 stars
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anti h3k27ac  (Rockland Immunochemicals)
92
Rockland Immunochemicals anti h3k27ac
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Anti H3k27ac, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+K27ac+Antibody/pmc06463023-92-3-4
Average 92 stars, based on 1 article reviews
anti h3k27ac - by Bioz Stars, 2026-11
92/100 stars
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histone h3 ps28 pe antibody  (Miltenyi Biotec)
94
Miltenyi Biotec histone h3 ps28 pe antibody
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Histone H3 Ps28 Pe Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+pS28+Antibody%2C+anti-human%2Fmouse%2C+REAfinity/pm41807778-33-5-28
Average 94 stars, based on 1 article reviews
histone h3 ps28 pe antibody - by Bioz Stars, 2026-11
94/100 stars
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rabbit polyclonal anti histone h3 3  (Novus Biologicals)
92
Novus Biologicals rabbit polyclonal anti histone h3 3
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Rabbit Polyclonal Anti Histone H3 3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3%2E3+Antibody/pmc08071442-35-58-63
Average 92 stars, based on 1 article reviews
rabbit polyclonal anti histone h3 3 - by Bioz Stars, 2026-11
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h3k4me2 reduction  (MedChemExpress)
92
MedChemExpress h3k4me2 reduction
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
H3k4me2 Reduction, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+(tri+methyl+K9)+Antibody/pm38516819-280-13-55
Average 92 stars, based on 1 article reviews
h3k4me2 reduction - by Bioz Stars, 2026-11
92/100 stars
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β actin  (Rockland Immunochemicals)
90
Rockland Immunochemicals β actin
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
β Actin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3+K27me3+Antibody/pmc08062036-992-17-16
Average 90 stars, based on 1 article reviews
β actin - by Bioz Stars, 2026-11
90/100 stars
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nbp2 67530  (Novus Biologicals)
94
Novus Biologicals nbp2 67530
Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone <t>H3;</t> CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.
Nbp2 67530, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3%2E3+Antibody+(ST50-08)/pm41872193-257-82-83
Average 94 stars, based on 1 article reviews
nbp2 67530 - by Bioz Stars, 2026-11
94/100 stars
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nbp2  (Novus Biologicals)
93
Novus Biologicals nbp2

Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h3%2E3+antibody/Histone+H3%2E3+Antibody/pmc09121328-17-7-3
Average 93 stars, based on 1 article reviews
nbp2 - by Bioz Stars, 2026-11
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Image Search Results


Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone H3; CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.

Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Article Title: Colchicine prevents perioperative myocardial injury in cardiac surgery by inhibiting the formation of neutrophil extracellular traps: evidence from rat models.

doi: 10.1093/ejcts/ezae364

Figure Lengend Snippet: Figure 3: NETs release increased in the perioperative period of heart surgery. (A) HE staining assessed inflammatory cell infiltration in myocardial tissues from sham, CPB and I/R rats. (B) TUNEL staining (scale bars, 20 µm) and immunofluorescence for CD11c, MPO and NE (scale bars, 20 µm) evaluated apoptosis and protein ex pression levels. (C) Apoptosis is represented as the ratio of TUNEL-positive cells to total cells (n ¼ 6). (D and E) Fluorescence intensity of CD11c, MPO and NE was semi-quantitatively analysed (n ¼ 6). (F and G) Western blot quantified MPO, NE, cTNT and CitH3 levels in myocardial tissues (n ¼ 6). ‘ns’ denotes no significant dif ference. P < 0.01, P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated histone H3; CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; HE: haematoxylin and eosin; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.

Article Snippet: Immunofluorescence Primary antibodies used were MPO (22225-1-AP, Proteintech, USA), NE (27642-1-AP, Proteintech, USA), cardiac troponin T (cTnT; 15513-1-AP, Proteintech, USA), CD11c (81853-1-RR, Proteintech, USA), citrullinated histone H3 (13754-1-AP, Proteintech, USA) and PAD-4 (17373-1-AP, Proteintech, USA).

Techniques: Staining, TUNEL Assay, Immunofluorescence, Fluorescence, Western Blot, Standard Deviation

Figure 4: Colchicine reduced NETs release in the perioperative period of heart surgery. (A) HE staining evaluated inflammatory cell infiltration in colchicine-treated and control rats. (B) Immunofluorescence for CD11c, cTNT, CitH3, MPO and NE (scale bars, 20 μm) analysed protein expression levels. (C) Fluorescence intensity of these markers was semi-quantitatively assessed (n ¼ 6). (D) Western blot quantified cTNT and CitH3 levels (n¼6). ‘ns’ denotes no significant difference. P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated Histone H3; COL: colchicine; CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; I/R: ischaemia–reperfu sion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.

Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Article Title: Colchicine prevents perioperative myocardial injury in cardiac surgery by inhibiting the formation of neutrophil extracellular traps: evidence from rat models.

doi: 10.1093/ejcts/ezae364

Figure Lengend Snippet: Figure 4: Colchicine reduced NETs release in the perioperative period of heart surgery. (A) HE staining evaluated inflammatory cell infiltration in colchicine-treated and control rats. (B) Immunofluorescence for CD11c, cTNT, CitH3, MPO and NE (scale bars, 20 μm) analysed protein expression levels. (C) Fluorescence intensity of these markers was semi-quantitatively assessed (n ¼ 6). (D) Western blot quantified cTNT and CitH3 levels (n¼6). ‘ns’ denotes no significant difference. P < 0.001. Data are expressed as mean (SD). CitH3: citrullinated Histone H3; COL: colchicine; CPB: cardiopulmonary bypass; cTNT: cardiac troponin T; I/R: ischaemia–reperfu sion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extracellular traps; SD: standard deviation.

Article Snippet: Immunofluorescence Primary antibodies used were MPO (22225-1-AP, Proteintech, USA), NE (27642-1-AP, Proteintech, USA), cardiac troponin T (cTnT; 15513-1-AP, Proteintech, USA), CD11c (81853-1-RR, Proteintech, USA), citrullinated histone H3 (13754-1-AP, Proteintech, USA) and PAD-4 (17373-1-AP, Proteintech, USA).

Techniques: Staining, Control, Immunofluorescence, Expressing, Fluorescence, Western Blot, Standard Deviation

Figure 5: Suppressing PAD-4 protein significantly reduced NETs release. (A) Immunofluorescence (scale bars, 20 μm) analysed PAD-4, MPO, NE, cTNT and CitH3 lev els in I/R þ CI-am and I/R þ saline groups. (B) Fluorescence intensity of CD11c, cTNT, CitH3, MPO and NE was semi-quantitatively analysed (n ¼ 6). (C–E) Western blot quantified PAD-4, MPO, NE, cTNT and CitH3 levels (n ¼ 6). ‘ns’ denotes no significant difference. P < 0.001. Data are expressed as mean (SD). CI-am: CI-ami dine; CitH3: citrullinated histone H3; cTNT: cardiac troponin T; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extra cellular traps; PAD-4: peptidylarginine deiminase 4; SD: standard deviation.

Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Article Title: Colchicine prevents perioperative myocardial injury in cardiac surgery by inhibiting the formation of neutrophil extracellular traps: evidence from rat models.

doi: 10.1093/ejcts/ezae364

Figure Lengend Snippet: Figure 5: Suppressing PAD-4 protein significantly reduced NETs release. (A) Immunofluorescence (scale bars, 20 μm) analysed PAD-4, MPO, NE, cTNT and CitH3 lev els in I/R þ CI-am and I/R þ saline groups. (B) Fluorescence intensity of CD11c, cTNT, CitH3, MPO and NE was semi-quantitatively analysed (n ¼ 6). (C–E) Western blot quantified PAD-4, MPO, NE, cTNT and CitH3 levels (n ¼ 6). ‘ns’ denotes no significant difference. P < 0.001. Data are expressed as mean (SD). CI-am: CI-ami dine; CitH3: citrullinated histone H3; cTNT: cardiac troponin T; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; NETs: neutrophil extra cellular traps; PAD-4: peptidylarginine deiminase 4; SD: standard deviation.

Article Snippet: Immunofluorescence Primary antibodies used were MPO (22225-1-AP, Proteintech, USA), NE (27642-1-AP, Proteintech, USA), cardiac troponin T (cTnT; 15513-1-AP, Proteintech, USA), CD11c (81853-1-RR, Proteintech, USA), citrullinated histone H3 (13754-1-AP, Proteintech, USA) and PAD-4 (17373-1-AP, Proteintech, USA).

Techniques: Immunofluorescence, Saline, Fluorescence, Western Blot, Standard Deviation

Figure 7: Colchicine reduced NETs release by suppressing PAD-4. (A) TUNEL staining (scale bars, 20 μm) assessed myocardial apoptosis in I/R þ COL and I/R þ CI- am groups. Immunofluorescence (scale bars, 20 μm) evaluated PAD-4, MPO, NE, cTNT and CitH3 levels. (B) Apoptosis and protein levels were semi-quantitatively analysed (n ¼ 6). (C) Western blot quantified PAD-4, MPO, NE, cTNT and CitH3 levels. ‘ns’ denotes no significant difference. P<0.001. Data are expressed as mean (SD). CI-am: CI-amidine; Cith3: citrullinated histone H3; COL: colchicine; cTNT: cardiac troponin T; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; PAD-4: peptidylarginine deiminase 4; SD: standard deviation.

Journal: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

Article Title: Colchicine prevents perioperative myocardial injury in cardiac surgery by inhibiting the formation of neutrophil extracellular traps: evidence from rat models.

doi: 10.1093/ejcts/ezae364

Figure Lengend Snippet: Figure 7: Colchicine reduced NETs release by suppressing PAD-4. (A) TUNEL staining (scale bars, 20 μm) assessed myocardial apoptosis in I/R þ COL and I/R þ CI- am groups. Immunofluorescence (scale bars, 20 μm) evaluated PAD-4, MPO, NE, cTNT and CitH3 levels. (B) Apoptosis and protein levels were semi-quantitatively analysed (n ¼ 6). (C) Western blot quantified PAD-4, MPO, NE, cTNT and CitH3 levels. ‘ns’ denotes no significant difference. P<0.001. Data are expressed as mean (SD). CI-am: CI-amidine; Cith3: citrullinated histone H3; COL: colchicine; cTNT: cardiac troponin T; I/R: ischaemia–reperfusion; MPO: myeloperoxidase; NE: neutrophil elastase; PAD-4: peptidylarginine deiminase 4; SD: standard deviation.

Article Snippet: Immunofluorescence Primary antibodies used were MPO (22225-1-AP, Proteintech, USA), NE (27642-1-AP, Proteintech, USA), cardiac troponin T (cTnT; 15513-1-AP, Proteintech, USA), CD11c (81853-1-RR, Proteintech, USA), citrullinated histone H3 (13754-1-AP, Proteintech, USA) and PAD-4 (17373-1-AP, Proteintech, USA).

Techniques: TUNEL Assay, Staining, Immunofluorescence, Western Blot, Standard Deviation

Journal: iScience

Article Title: Nde1 is required for heterochromatin compaction and stability in neocortical neurons

doi: 10.1016/j.isci.2022.104354

Figure Lengend Snippet:

Article Snippet: Histone H3.3 , Novus Biologicals , Cat# NBP2-24697.

Techniques: Ubiquitin Proteomics, Recombinant, Protease Inhibitor, Hybridization, Labeling, Plasmid Preparation, Software